A gene X coded by yeast cells encodes an RNA of 1350 nucleotides. The
corresponding protein for X (X-P) is 35 kDa.
(1) How would you detect the gene in the genome of yeast, the RNA for X
and the protein for X? Use sketches and bullet points. No details such as
method of gel setting, probe synthesis etc. is necessary. (3+3+3)
(2) While detecting the RNA you found that there are three radioactive
bands corresponding to the probe. The sizes of these bands are:
1500 nt, 1350 nt and 900 nt. What will be your conclusions? (3+3+3)
(3) While detecting the protein you found that there are three bands for
protein X-P at sizes 37 kDa, 35 kDa and 25 kDa. What will be your
conclusions? (4+1.5+1.5)
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