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Medical Questions
Your Question
1.why aren\'t the magnification of both ocular lenses of a binocular microscope used to calculate total magnification
2. why is wavelength the main factor on limit of resolution in light microscopy?
3. on a given microscope, the numerical apertures of the condenser and how low power objective lenses are 1.25 and 0.25, respectively. ou are supplied with a filter that selects a wavelength of 520nm.
a. what is the limit of resolution on this microscope?
b. will you be able to distinguish two points that are 330nm apart as being separate, or will they blur into one?
4.on the same microscope as in question 3, the high dry objective lens has a numerical aperure of 0.85.
a. what is the limit of resolution on this microscope?
b. will you be able to distinguish two points that are 250nm apart as being separate, or will they blur into one?
5. calculate the limit resolution for the oil lens of your microscope. Assume an average wavelength of 500nm
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